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Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.

Abstract:

Cdt1 plays a critical role in DNA replication regulation by controlling licensing. In Metazoa, Cdt1 is regulated by CRL4(Cdt2)-mediated ubiquitylation, which is triggered by DNA binding of proliferating cell nuclear antigen (PCNA). We show here that fission yeast Cdt1 interacts with PCNA in vivo and that DNA loading of PCNA is needed for Cdt1 proteolysis after DNA damage and in S phase. Activation of this pathway by ultraviolet (UV)-induced DNA damage requires upstream involvement of nucleoti...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkr222

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
Edward Penley Abraham Cephalosporin Fund More from this funder
Cancer Research UK More from this funder
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic acids research Journal website
Volume:
39
Issue:
14
Pages:
5978-5990
Publication date:
2011-08-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048

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