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A modified density gradient proteomic-based method to analyze endolysosomal proteins in cardiac tissue

Abstract:

The importance of lysosomes in cardiac physiology and pathology is well established, and evidence for roles in calcium signaling is emerging. We describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endolysosomal (EL) proteins. Density gradient fractions corresponding to tissue lysate; sarcoplasmic reticulum (SR), mitochondria (Mito) (1.3 g/mL); and EL with negligible contamination from SR or Mito (1....

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.isci.2021.102949

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Role:
Author
ORCID:
0000-0003-4432-5491
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Institution:
University of Oxford
Division:
MSD
Sub department:
Pharmacology
Role:
Author
ORCID:
0000-0003-2922-3075
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Role:
Author
ORCID:
0000-0002-7422-589X
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Role:
Author
ORCID:
0000-0002-0030-0148
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Publisher:
Elsevier Publisher's website
Journal:
iScience Journal website
Volume:
24
Issue:
9
Article number:
102949
Publication date:
2021-09-24
Acceptance date:
2021-08-02
DOI:
EISSN:
2589-0042
Language:
English
Keywords:
Pubs id:
1190263
Local pid:
pubs:1190263
Deposit date:
2021-08-10

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