Journal article
A modified density gradient proteomic-based method to analyze endolysosomal proteins in cardiac tissue
- Abstract:
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The importance of lysosomes in cardiac physiology and pathology is well established, and evidence for roles in calcium signaling is emerging. We describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endolysosomal (EL) proteins. Density gradient fractions corresponding to tissue lysate; sarcoplasmic reticulum (SR), mitochondria (Mito) (1.3 g/mL); and EL with negligible contamination from SR or Mito (1....
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, 3.2MB)
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- Publisher copy:
- 10.1016/j.isci.2021.102949
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Authors
Funding
Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- iScience Journal website
- Volume:
- 24
- Issue:
- 9
- Article number:
- 102949
- Publication date:
- 2021-09-24
- Acceptance date:
- 2021-08-02
- DOI:
- EISSN:
-
2589-0042
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
1190263
- Local pid:
- pubs:1190263
- Deposit date:
- 2021-08-10
Terms of use
- Copyright holder:
- Ayagama et al.
- Copyright date:
- 2021
- Rights statement:
- ©2021 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Licence:
- CC Attribution (CC BY)
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