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Averaging of viral envelope glycoprotein spikes from electron cryotomography reconstructions using Jsubtomo.

Abstract:

Enveloped viruses utilize membrane glycoproteins on their surface to mediate entry into host cells. Three-dimensional structural analysis of these glycoprotein 'spikes' is often technically challenging but important for understanding viral pathogenesis and in drug design. Here, a protocol is presented for viral spike structure determination through computational averaging of electron cryo-tomography data. Electron cryo-tomography is a technique in electron microscopy used to derive three-dime...

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Publisher copy:
10.3791/51714

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Publisher:
Journal of Visualized Experiments
Journal:
Journal of visualized experiments : JoVE
Issue:
92
Pages:
e51714
Publication date:
2014-01-01
DOI:
EISSN:
1940-087X
ISSN:
1940-087X
Source identifiers:
487909
Language:
English
Keywords:
Pubs id:
pubs:487909
UUID:
uuid:d45dc0d1-aa8c-46a4-965f-d36d3b923ce4
Local pid:
pubs:487909
Deposit date:
2014-10-29

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