Journal article icon

Journal article

Optogenetic control of iPS cell‐derived neurons in 2D and 3D culture systems using channelrhodopsin‐2 expression driven by the synapsin‐1 and calcium‐calmodulin kinase II promoters

Abstract:

Development of an optogenetically controllable human neural network model in three‐dimensional (3D) cultures can provide an investigative system that is more physiologically relevant and better able to mimic aspects of human brain function. Light‐sensitive neurons were generated by transducing channelrhodopsin‐2 (ChR2) into human induced pluripotent stem cell (hiPSC) derived neural progenitor cells (Axol) using lentiviruses and cell‐type specific promoters. A mixed population of human iPSC‐de...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Files:
Publisher copy:
10.1002/term.2786

Authors


More by this author
Role:
Author
ORCID:
0000-0002-2503-2338
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
ORCID:
0000-0002-0701-5040
More by this author
Role:
Author
ORCID:
0000-0002-9055-1775
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
Oxford college:
Linacre College
Role:
Author
ORCID:
0000-0001-7613-6041
More by this author
Role:
Author
ORCID:
0000-0001-9066-0181
Expand authors...
Ministry of Higher Education, Malaysia More from this funder
Publisher:
Wiley Publisher's website
Journal:
Journal of Tissue Engineering and Regenerative Redicine Journal website
Volume:
13
Issue:
3
Pages:
369-384
Publication date:
2019-01-30
Acceptance date:
2018-11-30
DOI:
EISSN:
1932-7005
ISSN:
1932-6254
Pmid:
30550638
Source identifiers:
955104
Language:
English
Keywords:
Pubs id:
pubs:955104
UUID:
uuid:d66ade4d-3363-4d99-89f5-1c1185da2b8d
Local pid:
pubs:955104
Deposit date:
2019-01-31

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP