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Measurement of T cell alloreactivity using imaging flow cytometry

Abstract:

The measurement of immunological reactivity to donor antigens in transplant recipients is likely to be crucial for the successful reduction or withdrawal of immunosuppression. The mixed leukocyte reaction (MLR), limiting dilution assays, and trans-vivo delayed-type hypersensitivity (DTH) assay have all been applied to this question, but these methods have limited predictive ability and/or significant practical limitations that reduce their usefulness. Imaging flow cytometry is a technique tha...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3791/55283

Authors


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Role:
Author
ORCID:
0000-0002-7243-556X
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Surgical Sciences
Role:
Author
ORCID:
0000-0002-7466-3849
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Institution:
University of Oxford
Division:
MSD
Department:
Surgical Sciences
Oxford college:
Green Templeton College
Role:
Author
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International Society for Heart and Lung Transplantation Research More from this funder
Royal College of Physicians and Surgeons of Canada More from this funder
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Publisher:
Journal of Visualised Experiments Publisher's website
Journal:
Journal of Visualized Experiments Journal website
Volume:
2017
Issue:
122
Publication date:
2017-04-19
DOI:
EISSN:
1940-087X
Pmid:
28448002
Source identifiers:
691410

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