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Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition

Abstract:

Despite tremendous advances in sample preparation and classification algorithms for electron cryomicroscopy (cryo-EM) and single-particle analysis (SPA), sample heterogeneity remains a major challenge and can prevent access to high-resolution structures. In addition, optimization of preparation conditions for a given sample can be time consuming. In the current work, it is demonstrated that native electrospray ion-beam deposition (native ES-IBD) is an alternative, reliable approach for prepar...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/pnasnexus/pgac153

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Publisher:
Oxford University Press Publisher's website
Journal:
PNAS Nexus Journal website
Volume:
1
Issue:
4
Article number:
pgac153
Publication date:
2022-08-06
Acceptance date:
2022-08-03
DOI:
EISSN:
2752-6542

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