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Factor inhibiting HIF can catalyse two asparaginyl-hydroxylations in VNVN motifs of ankyrin fold proteins

Abstract:

The aspariginyl-hydroxylase human Factor Inhibiting HIF (FIH) is an important regulator of the transcriptional activity of hypoxia inducible factor (HIF). FIH also catalyses the hydroxylation of asparaginyl and other residues in ankyrin repeat domain (ARD) containing proteins, including apoptosis stimulating of p53 protein (ASPP) family members. ASPP2 is reported to undergo a single FIH catalysed hydroxylation at Asn-986. We report biochemical and crystallographic evidence showing FIH catalys...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.jbc.2022.102020

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Publisher:
Elsevier Publisher's website
Journal:
Journal of Biological Chemistry Journal website
Volume:
298
Issue:
6
Article number:
102020
Publication date:
2022-05-07
Acceptance date:
2022-04-15
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
Pmid:
35537551

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