Thesis
Single-molecule studies of transcription initiation
- Abstract:
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Single-molecule Förster resonance energy transfer (smFRET) has emerged as an important tool for studying biological reactions. This thesis describes smFRET investigations into the mechanism of bacterial transcription initiation.
We developed protocols to immobilize RNAP-DNA initiation complexes using vesicles and antibodies. We used these techniques to show that the transcription bubble conformation in immobilized complexes exhibits inter-molecular heterogeneity. We observed large F...
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Funding
+ Engineering and Physical Sciences Research Council
More from this funder
Funding agency for:
Duchi Llumigusin, D
Bibliographic Details
- Publication date:
- 2014
- Type of award:
- DPhil
- Level of award:
- Doctoral
- Awarding institution:
- Oxford University, UK
Item Description
- Language:
- English
- Keywords:
- Subjects:
- UUID:
-
uuid:fa5d7117-4270-4362-95f4-ce1c870f2921
- Local pid:
- ora:11394
- Deposit date:
- 2015-05-08
Terms of use
- Copyright holder:
- Diego Duchi
- Copyright date:
- 2014
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