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Thesis

Single-molecule studies of transcription initiation

Abstract:

Single-molecule Förster resonance energy transfer (smFRET) has emerged as an important tool for studying biological reactions. This thesis describes smFRET investigations into the mechanism of bacterial transcription initiation.

We developed protocols to immobilize RNAP-DNA initiation complexes using vesicles and antibodies. We used these techniques to show that the transcription bubble conformation in immobilized complexes exhibits inter-molecular heterogeneity. We observed large F...

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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Oxford college:
St Cross College
Role:
Author

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Role:
Supervisor
Publication date:
2014
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
Oxford University, UK
Language:
English
Keywords:
Subjects:
UUID:
uuid:fa5d7117-4270-4362-95f4-ce1c870f2921
Local pid:
ora:11394
Deposit date:
2015-05-08

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